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The SLAF sequences and the genotype of all mapping samples were given in Additional file 1.
Peptide mapping samples were loaded onto an Agilent SB-C18 2.1 mm × 150 mm column with 100% mobile phase A (0.05% TFA in 98% HPLC H2O/2% acetonitrile) and eluted with a gradient of 0.5%/min with mobile phase B (0.04% TFA in acetonitrile) over 68 min. The flow rate was 0.2 mL/min, and column temperature was set to 50 °C.
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The purpose of maximization of J X) is to find the mapping direction, and the final total divergence of mapping sample is the largest.
A change that was present in only one of the mutant plants as compared to a uniform genotype for the rest of the mapped samples was considered to be EMS induced after application of a threshold of GQ ≥ 30.
In order to develop the application rate map, 21 samples were previously taken for analysis of soil fertility.
The elemental analysis and maps of samples were obtained by the energy-dispersive X-ray spectroscope (EDS) system (Thermo Fisher, Noran System 7, United States of America), operated under vacuum.
Effective minority carrier lifetime maps of samples were measured after annealing using the microwave-detected photoconductive decay (μ-PCD) WT-20000, Semilab).
As for proteome map construction, samples were taken during the late fed-batch production phase (around 160 h) for each sample.
Genes with an average of at least one uniquely mapped read across samples were tested for differential expression using QuasiSeq (http://cran.r-project.org/web/packages/QuasiSeq).org/web/packages/QuasiSeq
L* values of coated + MAP and control samples were 68.8 and 48.9 after 8 days storage, respectively.
Those transcripts with no reads mapped in all samples were considered errors and removed.
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