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The second caveat is related to uncertainty in mapping reads to specific TE copies due to the repetitive nature to TEs.
Software is available for MIP design47 and standard or dedicated48 tools may be used for mapping reads to reference and calling polymorphisms.
A conservative set of SNPs was called for each sample in the training set (St_A1) and validation set (St_B1) by first mapping reads to the MRSA252 reference genome with Stampy61, and then calling variants with samtools62.
A large number of sequence alignment tools can be found in literature which are very efficient and powerful in mapping reads to the reference genome.
Hence, neither Bowtie nor MAQ should be used for mapping reads to quantify gene expression.
Gene expression profiling was measured by mapping reads to assembled sequences using SOAP [66].
TopHat allows multiple alignments per read (up to 40 by default) and a maximum of 2 mismatches when mapping reads to the reference.
First, SMS produces reads that are, on average, shorter than their AS counterparts, magnifying the issue of accurately mapping reads to their correct positions.
Sequence heterogeneity between alleles was great enough to produce poor performance by ELAND in mapping reads to the genome, so this mapping was performed with the NCBI BLAST program (Table S1.1).
To test this, we re-mapped reads that match multiple genomic loci (group R) to the regions of low-coverage, this time randomly assigning ambiguously mapping reads to one of their genomic matches.
Therefore, mapping reads to a reference genome has two limitations.
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