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Figure 3 shows the relationship between the mapping ratio and the species classification.
In summary, SCRS data amplified by MDA or MALBAC had a lower duplication ratio, a higher mapping ratio, and a higher genome recovery than that from DOP-PCR.
Therefore, increasing the numbers of mismatches and gaps will contribute to increasing the mapping ratio and subsequent biological analyses, even if the method is probabilistic.
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Pearson correlation between mapping ratios and genetic distance.
The relationship between the mapping ratios and genetic distance from reads to reference genome were examined by Pearson correlation.
First, we evaluated the mapping ratio, duplication ratio, and genome coverage uniformity using the single-cell low-coverage whole genome sequencing (LWGS) data or the extracted single-cell LWGS data.
Finally, clean reads were re-mapped to the draft cp genomes of two Phoebe species, and the mapping ratio were 3.41% for P. chekiangensis and 2.15% for P. bournei, and average coverage depth were 578.2 and 367.1 for P. chekiangensis and P. bournei, respectively.
The average total number of bases and mapping ratio for the 12 samples were 101 GB and 95.7%, respectively (Table 1).
To investigate relationships between the nucleotide identity and mapping ratio, the nucleotide identity between a given FLcDNA and the genomic locus to which it was assigned by est2genome was calculated.
The number of clean reads and mapping ratio are presented in the supplemental material (Additional file 3: Table S3), and the mapping ratios were all more than 90%, which suggests that sequencing was sufficient.
Number of clean reads and mapping ratio in three A. niger strains.
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