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Mapping rates obtained for the P. patula transcriptome were similar to the mapping rates obtained for the P. radiata and P. taeda transcriptome assemblies.
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Short reads were mapped to contigs by Bowtie2 [ 36], and mapping rates were obtained from its summary output.
Only the survival rates obtained with the p38 MAP Kinase inhibitor SCIO-469 (which were three times increased) were superior to these results (Vanderkerken et al, 2007).
According to the average error rate obtained from a pilot mapping, we decided to keep the first 42 bp of each read and ran the mapping again.
We obtained mapping rates to the reference genome of only ~13% of the adult reads and <4% of the embryonic reads (Marco et al., 2010).
P. palustris is most closely related to loblolly pine and obtains mapping rates similar to the reclustered ESTs at 71.1% with 95% query coverage and 95% identity.
Examining the overall mapping rates, we see that GSNAP obtains the lowest mapping rates, ranging from 0.4497 to 0.4857.
When mapping to the reference transcriptome, Bowtie2 obtains slightly higher mapping rates of 0.6269 to 0.7052.
In this study TopHat2 obtains significantly higher mapping rates ranging from 0.5940 to 0.6365.
Bowtie2 mapping to the reference genome (Bowtie2-G) obtains higher still mapping rates of 0.6227 to 0.6530.
Overall, 22-69 million uniquely mapped reads with mapping quality higher than or equal to 20 were obtained for these samples and their mapping rates ranged from 72%to90%0%.
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CEO of Professional Science Editing for Scientists @ prosciediting.com