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We analyzed two real phenotypes (hair length and furnishing), that are fixed in certain breeds to characterize mapping precision of the known causal mutations.
Given the fixation status of these traits, these well-defined phenotypes are relevant to investigate the mapping precision of an EG-GWAS approach across breeds.
Wu, Y., Zheng, Z., Visscher, P. M. & Yang, J. Quantifying the mapping precision of genome-wide association studies using whole-genome sequencing data.
We determine how, for a fixed total electron-budget, the available dose should be fractionated for maximum strain mapping precision.
Finally, using real phenotypes, the mapping precision of EG-GWAS to identify variants inside the RSPO2 and FGF5 genes, associated with two coat types in dogs was investigated15,16.
We successfully tested our method by mapping five known genes to a precision of 0.2 24 kb (0.1 10 cM), and developed computer simulations to test the effect of different factors on mapping precision.
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The software also calculates a measure of map precision.
Here, we performed equivalent virtual injections on simulated maps to assess map precision.
The difference in map precision can also be seen in the projection on the NT axis onto the AP axis [ Fig. 4(A D)].
Although all soybean sequences were sequentially placed in relation to the A. thaliana genome, a limitation on map precision means that separation into uniquely delimited map entries does not occur within a 400 bp range.
To further characterize map precision, paired dye injections were made into SC to see how the retrogradely transported labels separated in the retina (Upton et al., 2007; Lyngholm et al., 2013).
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