Exact(3)
A piRNA was considered overlapping with an ME if its mapping position is entirely within an ME.
The mapping results were processed with SAMtools [ 40] to extract for each transcript the number of mapped reads and determine, whether their mapping position is unique.
Moreover, an aligned read is deemed to be correctly aligned only if the mapping position is identical to the true position of the read.
Similar(5)
In summary, using a distant reference transcriptome resulted in a) fewer tags mapping, b) some genes not being surveyed for differential expression and c) lower than expected levels of expression for genes whose most abundant mapping position was not conserved.
The log-likelihood ratio test statistic for a QTL at a particular map position is displayed graphically to generate a likelihood map or profile.
There remain the potential pitfalls that non specific signals are misinterpreted as specific if a putative map position is consistent with expectation, or that specific signals are not identified due to unexpected chromosomal aberrations.
A second male sterility gene ps2 has been reported, although its map position is unknown.
Once a genetic map position is obtained by recombination analysis, we use File S1 to estimate the corresponding physical location.
Related(2)
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