The positions of the mapped SSR loci from the three mapping populations were highly consistent with the physical map positions, with few exceptions (Fig. 7).
Three bi-parental mapping populations were used to develop the genetic map for organizing scaffolds into pseudomolecules.
The genotypic data for the two mapping populations were downloaded from http://ricediversity.org/data.org/data
Construction of a linkage map and QTL analysis for the three mapping populations were performed by using the R/qtl software (http://www.rqtl.org; Broman et al. 2003).
Their donor's parents as well as their genetic position and their co-localized markers in various mapping populations were also reported here.
The parental lines of two mapping populations were exposed to a pulse stress of 1,000 mg L−1 Fe2+ in hydroponics.
In 12 out of the 14 studies, biparental mapping populations were used and in two studies germplasm collections were used for genetic characterization of Zn concentrations.
Two pairs of parents from mapping populations were screened initially for tolerance to an Fe pulse stress at the vegetative growth stage: IR29/Pokkali, and Nipponbare/Kasalath.
Figure 1 Parents of two mapping populations were screened after a pulse stress of 1,000 mg L −1 Fe 2+ for 5 days.
The preferred mapping populations were recombinant inbred lines (RILs) or doubled haploid populations because the fixed nature of these lines enabled replicate measurements.
Two mapping populations were used for linkage mapping in this study.
