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In recent years, rice scientists conducted a series of linkage analyses using several biparental mapping populations to identify the quantitative trait loci (QTLs) associated with tolerance to flooding during anaerobic germination.
We used these two mapping populations to dissect the genetic architecture of salt tolerance in A. thaliana.
The first utilizes biparental mapping populations to monitor the co-segregation of QTL and marker loci.
One path involves generating additional experimental mapping populations to narrow an initial, wide QTL support interval [ 1].
We used four B. rapa mapping populations to develop an integrated linkage map: CKDH, CRF2, PF2, and CSKF2.
Wenzl et al. [ 2] combined DArT with RFLP, SSR and STS from nine mapping populations to create a consensus map containing 2935 markers.
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After screening 920 (520 RAPD, 100 ISSR, 300 SSR) primers among the parental lines, 336 polymorphic markers were identified to be used for genotyping the mapping population to develop genetic linkage map.
A total of 1,236 F2 progeny, including 691 from race 1×race 3 and 545 from race 2×race 7, were produced to establish an expanded mapping population to refine the Avr1a interval.
For consistency, our group has also selected this mapping population to construct the first SSR linkage map [ 7].
Initially, a panel of 177 plants, randomly selected from the F2 mapping population, was used as an unselected mapping population to delimit the Scmv1 region.
We hope to utilize the mapping population to separate and quantify the effects of the each QTL contributor, if possible.
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