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However, even if the resolution on QTL position is clearly improved in GWAS compared with mapping populations, the number of possible candidate genes is still large and requires complementary evidence to determine the functional gene(s).
To compare the mean RDRs in three genotypes for the DNA marker closest to the significant QTL in the three mapping populations, the Tukey's multiple-comparison test function provided in JMP software version 7.0 (SAS Institute Japan, Tokyo, Japan) was used.
About 13% of the SNPs were polymorphic in both mapping populations, the remainder being polymorphic in one population but not the other.
This clearly shows that with RH mapping populations, the number of mappable markers can be increased several fold over conventional genetic mapping methods.
With respect to SNPs between the parental lines of the mapping populations the Oregon Wolfe barleys showed 62 SNP in 37 loci or genes, respectively.
While a common line, 'B73', was used as the maternal line across the 25 mapping populations, the paternal lines were all different.
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In the biparental mapping population, the anaerobic response index of the parents, Nipponbare and IR64, were 2.08 and 1.36 cm, respectively.
When these primer pairs are used with a mapping population, the number may increase.
Phenotyping for resistance to C. personatum was performed on the parents of the mapping population, the F1 hybrid, individuals composing the mapping population itself, and A. hypogaea cv.
Were inbreeding depression causing overrepresentation of I. fulva alleles in the BCIB mapping population, the same mechanism would not cause such distortion in the BCIF mapping population.
However, in the OWB doubled haploid mapping population, the only locus with a perfect association with inflorescence type is VRS1.
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