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LKM, MJT, OEO and RWM developed mapping populations, provided EST data, and revised the manuscript.
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The identification of the distribution of SD and the effect of genetic factors causing SD in genetic mapping populations provides an opportunity to survey the whole genome for new SD loci and their relationships to reproductive barriers.
Structured mapping populations provide key information on the degree of complexity in the genetic control of UV-B-induced cotyledon opening in Arabidopsis.
The use of a mapping population provides a method to verify detected polymorphisms because alternative alleles are expected to segregate in equal proportion (1 1) in a sample of megagametophytes.
These mapping resources have complementary strengths, with the maize intermated recombinant inbred populations providing high resolution via relatively small introgressed regions (Lee et al. 2002) and the nested association mapping population providing allelic comparisons to a reference allele across the subpopulations (Yu et al. 2008).
Indeed, tools such as molecular markers, high-density genetic maps and structured mapping of populations provide breeders with the ability to "simultaneously define gene action and breeding value at hundreds of loci distributed relatively uniformly across entire genomes" (Moose and Munn [2008]).
Linkage data for markers in the three mapping populations are provided in Additional file 3: Table S1, Additional file 4: Table S2 and Additional file 7: Table S4.
SNP mapping of two candidate R genes in both the F1 NA6 × AU6) and p150/112 mapping populations has provided possible candidates for the major gene crown rust resistance QTL (LpPc1) on LG2 [ 14].
Furthermore, most QTL in this study overlapped with regions previously identified in other studies using different mapping populations, which provides additional confidence in the validity of the results.
The map, together with the use of the array in other genetic mapping or association mapping populations, will provide the basis from which trait-based mapping can be carried out in the future.
Development of a high density integrated genetic map of B. napus derived from well established mapping populations will provide a superior tool for high resolution mapping and verification of DNA sequence contig order and orientation.
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