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Much of the potential for fine-mapping in crops has remained largely unrealized, because progress in mapping population design has failed to match rapid advances in genotyping technologies.
In addition, recent developments in genetic mapping population design widen the opportunities for genetic analyses in crops.
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DLH designed and oversaw the study, developed the mapping population, designed the GoldenGate OPA, performed genotyping analysis of the GoldenGate assay, and drafted the manuscript.
Most of the mapping populations designed to map drought tolerance QTL, derived from indica x japonica crosses, usually detect favorable alleles for drought-resistance traits contributed by japonica lines [ 20].
Two 15-plex SNPWave assays were designed and tested using two parental lines and 94 recombinant inbred lines (RIL) offspring of the ISU (B73×Mo17) maize mapping population.
Ninety primer pairs were designed and tested for their ability to detect polymorphism across a panel of 11 pairs of pearl millet mapping population parental lines.
In contrast, much higher rates of polymorphic SNPs can be achieved by transcriptome resequencing of parental genotypes in the target mapping population, allowing the design of customized OPAs containing SNPs that are segregating in the mapping pedigree.
To establish the mapping population, we used a North Carolina Design 3 (NCD3), in which individual F2 males were backcrossed to females from both parental lines.
For a mapping population with the particular breeding design, the realized junction densities and IBD probabilities are saved for all individuals in each generation in each simulation replicate, and they are averaged over in total 2 × 10 4 replicates.
Such heterogeneous recombination frequencies occurred because the present study incorporated data collected from several mapping populations differing in design, size and marker density.
SAP produced the mapping population, generated the phenotypic data, designed the genotyping assay, and generated the genotypic data.
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