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The mapping population, which consists of 114 F7:8 recombinant inbred lines (RILs) developed by single-seed descent from a cross between 'ZN016', a rustic landrace asparagus bean (Vigna. unguiculata ssp. sesquipedialis) variety originating from southern China and 'Zhijiang282', a commercial asparagus bean cultivar in China, as well as the mapping parents were used in this study.
In POP 2, male and female mapping parents were used in a combined 'HS' analysis.
Primers that were polymorphic between the mapping parents were used to genotype the 141 individuals of the BC1 population.
The haplotypes of the mapping parents were deduced at every linkage group using a custom-made VBA program.
To identify QTL-heterozygotes, individual mapping parents were tested for segregation of a major QTL at individual markers or haplotypes using a two-way chi-square test.
Finally, we report on levels of linkage disequilibrium (LD) between markers in the aquaculture strain from whence the mapping parents were sampled.
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For most markers, the parental phase in the Kanota and Ogle mapping parents was known, and +/- marker scores were converted to the scoring convention used by various software packages.
The selection of mapping parents is important for constructing high-density map.
The level of observed homozygosity in the mapping parents is shown in Figure 5.
Selection of mapping parents is important in order to establish F2 populations that allow successful causal gene identification by map-based cloning.
This deviation was explained by deviations in only two of the eight families (130×148 and 131×145) providing evidence that the mapping parents are relatively homogeneous within the sexes for recombination differences.
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