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This study was designed to evaluate several electromechanical mapping parameters for assessment of myocardial viability and inducible ischemia as defined by dipyridamole single-photon emission computed tomographic (SPECT) imaging at rest in patients with severe ischemic cardiomyopathy.
This success has been due to using highly stringent mapping parameters for subgenome-specific mapping.
The derived mapping parameters for each participant were then applied to that person's (inherently co-registered) fractional anisotropy image.
Details of sequenced reads and mapping parameters for each library can be seen in Additional file 1: Table S8.
We also optimised mapping parameters for SNP detections and confirmed that the 20% minimum variant threshold for detecting SNPs in pooled samples of four individuals was appropriate.
Finally, we validated the optimal set of mapping parameters for the Helicos tSMS platform using a simulation approach, showing that the suggested parameters preferentially lead to alignments at the correct location, while not significantly inflating the rate of spurious alignments.
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In this experiment, we run the assembly with the default mapping parameters allowing for a maximum of two mismatches and the maximum of ten hits for a read.
The applied mapping parameters allowed for up to two mismatches to reflect the allelic variation.
The following experiments were performed according to the specific set of mapping parameters recommended for each sequencing platform.
These mapping parameters allowed for the retention of high-quality reads containing the majority of differences at CpG dinucleotide locations only.
The proportion of reads that successfully mapped to the Hessian fly genome assembly was high in all four transcriptomes, ranging from 75%to80%0%, despite strict mapping parameters that allowed for only 2 bp mismatches to account for the high sequence similarity among recently duplicated chemosensory genes.
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