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The mapping panel developed for this study, the Porosus Mapping Panel, was therefore derived from a simple two-generation pedigree.
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Panels developed after a high level of cell irradiation (10 000 to 50 000 rads) are very useful for high resolution regional mapping studies but they require a characterization with a very large number of markers to be useful for genome-wide mapping studies [ 4].
Because progeny tests indicated that B6.SNVg does not dominantly affect the tumor multiplicity phenotype of B6- Apc Min, we have developed a mapping panel of SNV sites in this line.
A maize association mapping panel with 155 diverse inbred lines developed by Yang et al. [ 53] was used to find associations between the DNA polymorphisms in the two maize genes and grain yield components.
Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline.
Biparental populations developed from individuals with contrasting traits, selected from within the association mapping panel, can serve as association mapping validation tools.
The mapping panel consisted of two distinct sample groups.
The association mapping panel was characterized by strong population substructure.
Consequently, the Porosus Mapping Panel can be divided into two sub-panels: the Linkage Reference Panel and the QTL Resource Panel.
Eighty-three of the individuals from this panel were also present in the Linkage Mapping Panel.
All markers were mapped using DNA from a previously described meiotic mapping panel [ 40].
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