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An optimally informative zebrafish SNP genetic mapping panel and evaluation of the genetic ancestry of common laboratory strains are described in Figure S1, Figure S2, and Table S3.
The genomic DNA of the gametophyte clones of mapping panel and 2 parental gametophyte clones was extracted primarily with a modified cetyltriethylammnonium bromide (CTAB) method [ 74].
In this study, we used the mapping panel and markers from Voss et al. (2011) to perform genome-wide QTL scans for metamorphic timing QTL.
Of the 183 SNPs that were validated, 167 were polymorphic in the NCCCWA genetic mapping panel and 159 were mapped to chromosomes on the genetic map (Table 3).
Effort was made to determine the number of NCED and ZEP genes in the maize genome, to map the genes on the LHRF mapping panel and to determine their expression patterns in the kernel of both maize parental inbred lines.
Therefore, GWAS mapping could detect beneficial alleles from excellent germplasm with different genetic background in one mapping panel and obtain the fine map of association genomic regions and candidate genes by using sufficient density of SNP markers.
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Descriptions and characteristics of the two rainbow trout mapping panels, and two Atlantic salmon mapping panels used for the comparative studies have previously been provided [ 23].
We used existing hybrid mapping panels and a newly created hybrid cross to map the met1 genomic region and determine the effect of met1 on larval growth, metamorphic timing, and gene expression in the brain.
While various pseudogenes were originally attributed to chromosomes 1, 2, 10, 16, 20, and 22 by polymerase chain reaction analysis of monochromosomal mapping panels and by the use of fluorescence in situ hybridization (FISH), five different pseudogenes are now assigned to chromosomal regions 2p11 (two copies), 10p11, 16p11, and 22q11.
The genetic and phenotypic variation across the RI strains, combined with extensive collections of cumulative data available in extensive web-based databases including gene expression data, genetic mapping panels and phenotypic data [ 28] underlies the utility of these reference strains in such characterization.
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