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Rigorous mapping of splicing regulatory elements identified an ESS at the 5′ end of MADD exon 16, which mediates exon skipping via a core U/G GGG motif.
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The distribution of probes sets across each exon for some 28,800 genes allows the mapping of splice variants.
454 and RACE reads were mapped to the Vitis vinifera L. genome sequence using preliminary Blast searches and fine mapping of splice junctions was performed using SPIDEY [ 57] with default settings for plant sequences.
(Allowing for small differences, up to V bp, in the coordinates of introns and exons to account for imprecise mapping of splice junctions, has negligible effects on the comparison results).
Our analysis of microarray and RNA-sequencing data includes differential expression of splicing factors that may regulate AS, and a complete map of splicing isoforms.
The Human Genome Project created an initial map of splice variation more than a decade ago.
Filichkin, S. A. et al. Genome-wide mapping of alternative splicing in Arabidopsis thaliana.
Such a mapping of the 5' splice sites using the splice leader trapping method in T. brucei resulted in the discovery of nearly 2500 alternative splice events in a stage-regulated manner [ 23].
Tissue-specific splicing has been recognized as an important factor in defining cellular function [ 29]; however, at the present time, there are insufficient data to allow consistent mapping of individual splice variants to pathways.
The mapping of SL (spliced leader -removed TSS (red leader -removed S1) showed TSSt each of thred gene modelinesymbB1.v1.2.000535 on scaffold 31, symbB1.v1.2.020241 on scaffold 1693, symbB1.v1.2.028834 on scaffold 3094) predints two transcripts by SL trans-splicing (red arrows in Figure S1).
However, the combination TRIzol RNA/RiboZero RNA-seq underperformed with only 6,4% of the reads mapping to splice junctions.
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