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Genome-wide mapping of nucleosome positions and correlation of genome-wide nucleosomal remodeling with the changes in the gene expression can help us understanding gene regulation on genome level.
Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones.
The mapping of nucleosome positions at the OREs locus may help to clarify this issue and would be important to further our understanding of the role of chromatin remodeling in hypertonicity-induced gene expressions.
Genome-wide mapping of nucleosome positions of eukaryotic genomes is a prerequisite to understand the basic mechanism of chromosomal organization.
Large-scale mapping of nucleosome occupancy has been performed in Saccharomyces cerevisiae [ 10- 13], Caenorhabditis elegans [ 14, 15], Drosophila melanogaster [ 16], and human [ 9].
This genetic connection between S2P and chromatin biology led us to perform a genome-wide mapping of nucleosome position and occupancy by MNase-Seq.
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LeRoy, G. et al. Proteogenomic characterization and mapping of nucleosomes decoded by Brd and HP1 proteins.
In collaboration with David MacAlpine's lab (Duke University), we used genome-wide mapping of nucleosomes to demonstrate that nucleosome-depleted regions are a critical determinant of the sites bound by ORC (Fig. 2).
Mapping of nucleosomes in vivo using micrococcal nuclease demonstrates that deletion of FUN30 leads to changes of the chromatin structure at the boundary element.
A handful of experimental techniques have been developed for genome-wide mapping of nucleosomes.
This approach provides the most accurate possible measurement of nucleosome positioning and enables allele-specific mapping of nucleosomes.
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