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This new approach has the sensitivity to enable G4 mapping of functionally important transcripts at natural abundance levels.
While these approaches have particular and powerful benefits, they also all have drawbacks that limit their usefulness in the mapping of functionally activated circuits.
Based on spatial expression data alone, the results suggest that gene expression can improve the mapping of functionally related homologs (either orthologs or paralogs).
Here we describe in detail our protocols for detection of FTL expression in these mice and discuss the main variables which need to be considered in the use of these mice for the detection and mapping of functionally activated neurons, circuits and regions in the brain.
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As ChIP-Seq data on additional histone modifications under the same cell condition become available, this analysis could be repeated to generate an increasingly complete and accurate nucleosome map of functionally important regions.
In particular, brute force sequencing initiatives will result in a better mapping of the functionally important regions in the genome and experimental approaches, such as CRISPR/Cas [ 128] will provide the opportunity to extensively test the functional impact of genetic variants.
The results reveal a previously unknown set of NATs that prevails in this parasite, their differential regulation in disease conditions and mapping to functionally well annotated genes.
These association signals illustrate that the considerable majority of common genetic variants associated with human phenotypes map to functionally important regions of the genome that regulate gene expression [ 41].
Within bovine TLR2, polymorphisms at amino acid positions 227, 305 and 326 map to functionally important sites of TLR2 and should be considered as candidate SNPs for immune related traits in cattle.
This subsequent surface-based approach permitted the precise mapping and thickness measurement of functionally defined ROIs during allodynic neuropathic pain.
The view of heterochromatin as a genetic wasteland gradually changed with the mapping of a number of functionally important Drosophila genes to constitutive heterochromatin [e.g., [ 24- 31]].
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