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The genetic map reported in this study depicts the mapping of expressed genes.
Thus, only the best characterized protein coding sequences were studied and gene predictions, or models, based on the mapping of expressed sequences to genomes were not considered.
Highly multiplexed Illumina SNP arrays are efficient tools to enhance mapping of expressed genes, thereby improving the resolution and usefulness of a genetic linkage map [ 42, 48, 69- 73].
The design of customized highly multiplexed Illumina SNP arrays appears as an efficient procedure to enhance the mapping of expressed genes and make linkage maps more informative and powerful in such species with poorly known genomes.
Large-scale studies based on mapping of expressed sequence data on genomic sequences have produced estimates of as many as 30 60% of human genes undergoing alternative splicing [ 1- 5].
Many putative gene promoters have been identified either through mapping of expressed sequence tags (ESTs) to the genome (Acembly [ 20], ECGene [ 21]), through sequence conservation studies with other organisms [ 22] or de novo computational prediction (e.g., FirstEF [ 23], DragonGSF [ 24]).
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Then, we integrated these SNP data into linkage maps of expressed genes and illustrated the possibility to rapidly improve the density of existing genetic maps for spruce species.
We report the construction of a high-density linkage map of expressed genes for the heterozygous genome of Eucalyptus using Single Feature Polymorphism SFPP) markers.
Even though a female linkage map with 180 microsatellite markers has been reported [ 2], there remains a need for maps of expressed sequence tags (ESTs) and cDNAs to inform yellowtail genomic structure.
A linkage map of expressed sequence tag (EST -based markEST -basedish (Raphanus sativus L). was constructed using a low-cost and high-efficiency single-nucleotide polymarkersm (SNP) genotyping method named multiplex polymeradishhain Raphanus–mixed probe dot-blot analysativuseLoped in this study.
The SNP genotyping assays developed in this study enabled to map hundreds of expressed genes in the conifer Picea, which represent a large increase over any previous gene mapping effort in gymnosperms e.g. [ 19, 42, 41, 68- 71].
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