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The utility of the approach outlined in this paper is the ease with which one can increase resolution by increasing the number of primers in the 22q11 deleted region thus facilitating accurate mapping of deletion breakpoints.
Extensive experimental validation of this array has been described previously, using CGH (comparative genomic hybridization), mapping of deletion, specific PCR and quantitative RT-PCR [ 38, 64].
Again, as for the fine mapping of deletion breakpoints, the use of microsatellites did not facilitate a precise location of recombination breakpoints (or crossovers) (Fig. 4A).
Extensive experimental validation of this array has been described previously, using CGH, mapping of deletion and quantitative RT-PCR [ 33]. S. aureus strains were grown overnight in MHB, as described for proteomics analysis.
This S. aureus specific probe set together with control probes were used to manufacture an oligoarray that was extensively validated for comparative genomics, molecular epidemiology, mapping of deletion mutations, and transcription profiling applications.
A manufactured oligoarray including a subset of control Escherichia coli probes was validated for applications in the fields of comparative genomics and molecular epidemiology, mapping of deletion mutations and transcription profiling.
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The fine structure of qPCR mapping of deletions will reveal important clues into the mechanism by which the deletion occurs and thus will offer insights into the "at risk" factors predictive of deletions or other rearrangements.
Mapping of deletions and translocation breakpoints on 22q using high-resolution techniques revealed 22pter 22q11.2, 22q11, 22q11.21 12.2, and 22q13.1 13.3 to be the "hotspots" where the elusive tumor suppressor gene is likely to be found [55], [57]– [57].
Local physical maps of deletions are built allocating the deleted gene sequences and the syntenic genomic fragments from these other eudicots into a BES database of the species of interest.
Therefore, despite the great success in developing human genome maps of deletions and duplications, the mapping of inversions has lagged behind.
84, 85 Mapping of the deletion breakpoints in a cohort of Langer Giedion syndrome patients showed that 75% have cytogenetically detectable deletions of 8q24.1.
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