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Over 5,000 chosen SNPs are used to demonstrate high-resolution mapping of crossover points in the progeny of a historical sexual cross [ 21].
If the deletions in R1 and R2 cells occurred by homologous recombination, the nucleotide differences distributed along the entire length of uHS and dHS would allow the mapping of crossover sites into an interval between two polymorphic positions.
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More recently, fine-scale mapping of crossovers in Drosophila have found substantial variation in crossover rates at smaller scales (~5−50 kb) (Cirulli et al. 2007; Singh et al. 2009; Comeron et al. 2012; Singh et al. 2013).
A recent study in Saccharomyces cerevisiae used the array-based genotyping methodology presented here to create a genome-wide map of crossover and non-crossover gene conversion with the highest resolution to date (Mancera et al., 2008).
High-resolution genome-wide mapping of the crossover and non-crossover events from a large number of meioses in S. cerevisiae also shows that crossovers sometimes occur close to centromeres, but not within them, and that gene conversion does not occur close to centromeres at all [ 26].
While pseudo-testcross based analysis results in separate maps for each parent, the combined approach allows direct integration into a single map of crossovers that occur in either or both parents by using the markers that are heterozygous in both parents as anchors.
Tom Petes (Duke University, USA) described evidence that spontaneous mitotic crossovers in yeast are often initiated by a break in unreplicated DNA and presented genome-wide mapping of mitotic crossovers in yeast.
In our previous mapping of unselected crossovers on the right arm of chromosome IV (St. Charles and Petes 2013), the region containing the inverted Ty elements was associated with a high frequency of crossovers and was termed HS4 (Hot Spot4).
Mapping the location of crossovers in these backcross progeny provided information on the recombination events arising in the F1 hybrids.
Centromere mapping allows the development of improved linkage maps, deciphering of chromosome arms, investigation of crossover events and understanding of crossover interference during meiosis.
For each map, the distribution of crossovers mirrors those shown in Fig. 3.
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