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Mapping of conserved aa onto the structure revealed conservation of further sites and structural elements for each protein.
Macrosynteny may be revealed either by comparative genetic mapping of markers or, more recently, by physical mapping of conserved sequences within sequenced reference genomes [ 38, 39].
Similarly, mapping of conserved proteins against experimentally known IEDB epitopes identified six epitopes which had exact epitope match in four proteins including three from earlier predicted six PAPs.
This property contrasts with the surprising stability of lepidopteran chromosomes revealed by comparative mapping of conserved synteny in this and other recent studies [13], [14].
A multiple alignment of representative members from each cluster and mapping of conserved secondary structure information onto the sequences shows significant variation in the lengths of topologically equivalent β-strands containing the metal binding residues across functionally different cupins (Boxed in Figure 4).
Mapping of conserved and variable positions on PsbO surface also indicated new potential interaction regions.
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Intron loss was not addressed in the paper, this would require mapping the distribution of conserved introns onto a phylogenetic tree.
Additionally, CEGMA (Core Eukaryotic Genes Mapping Approach) analysis of conserved eukaryotic genes (CEGs), determined that our filtered transcriptome contained 453 full-length CEGs of the complete set of 458 CEGs (99%), and 238 of the more-conserved set of 248 CEGs (96%) [ 51, 52].
Also, mapping of the conserved gene ACTBL2 to an RMEL3 intron may represent a meaningful regulatory interrelationship with reflex in the actin cytoskeleton dynamics and cell migration.
Structural mapping of highly conserved positions again recovers the starting pattern, although a few β-sheet-associated glycines have been replaced by charges on the most exposed loops.
Mapping of the conserved residues onto the 3D-structure of an LPMO belonging to each family showed that the conserved region extends through the center of the proteins and protrudes on both sides of the structure.
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