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For T2 mapping of cells in vitro, the following parameters were used: point resolution of 156 × 156 μm, section thickness of 0.6 mm, TE = 20, 40, 60, 80, 100, 120, 140, 160 ms, TR = 4000 ms, number of acquisitions =2.
The SERS spectra and SERS mapping of cells indicate that the major Raman bands are mostly assigned to the vibration characteristics of proteins, and the C-H in-plane bending mode of the substituted benzene in Phenylalanine is remarkably enhanced.
Optical mapping of cells loaded with the Ca2+-sensitive fluorescent dye Fluo-4 AM via a complementary metal-oxide semiconductor camera ULTIMA-L (SciMedia, USA Ltd, Costa Mesa, CA) was used to monitor localized changes in [Ca2+] i.
Cre recombinase based fate mapping of cells expressing Phosphodiesterase 1c (Pde1c) revealed beta-galactosidase positive cells in the ventricular zone, as well as the subplate, suggesting that subplate neurons and cortical projection neurons may be derived from common progenitors.
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The mapping of cell morphology with the strain field revealed that the cells exposed to the equibiaxial strain exhibited the classical spindle morphology while the cells subjected to uniaxial strain changed to a polygonal morphology.
The goal of this study was to engineer cardiac tissue constructs with uniformly anisotropic architecture, and to evaluate their electrical function using multi-site optical mapping of cell membrane potentials.
The mapping of cell density to signal molecule concentration and the corresponding network modules involved have been at least partially characterized in many bacteria, and vary markedly between different systems.
In addition, we used a 2D mapping of cell shape to obtain the different shape changes associated with each strain energy component.
Our previous study in N1IP::CRE reporter mice, which permit the mapping of cell lineages experiencing Notch1 activation in vivo (Vooijs et al., 2007), illustrated that cells experiencing Notch1 activation never become NE cells (Morimoto et al., 2010).
IF is one of the best methods to unravel such complexity allowing fine mapping of cell wall composition at different levels of organisation, from organs to cell wall microdomains at any given developmental stage.
Beyond these STR markers, we developed a second set of single nucleotide polymorphism (SNP) markers to be used in the second stage of fine scale mapping of cell aggregation QTL by directly sequencing the relevant DNA regions.
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