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The high-resolution of aCGH tools used in our initial CNV screen [18] allowed precise mapping of boundaries.
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Automated base calling of the raw sequences for mapping of boundary ends of putative conserved miRNAs and removal of vector and adaptor sequences were performed with commercial software Sequencher 4.9.
When necessary, downloaded maps of boundaries of oil blocks and their attributes were digitized using ArcGIS 9.2.
Field samples of a subset of 30 rainforest patches supported the accuracy of the GIS-based mapping of rainforest boundaries.
While the overall patterns of DNA amplification and deletion corroborate previous cytogenetic studies, the high-resolution (gene-by-gene) mapping of amplicon boundaries and the quantitative analysis of amplicon shape provide significant improvement in the localization of candidate oncogenes.
While the carbon coating allowed precise mapping of the boundaries of the iron (in the mineral based pigment) in backscatter SEM mode, the dark black carbon coating visually obscured the paint pigment boundaries.
Mapping of geographic boundaries was conducted to locate all 33 communities and main roads.
Directional RNA-seq data were obtained that provide comprehensive mapping of transcript boundaries and abundance for all transcribed RNAs in Anabaena filaments during the response to nitrogen deprivation.
With recent whole genome mapping of gene boundaries, in the near future it may be possible to incorporate these regions into the transgenic construct design [ 21, 22].
In addition, the higher resolution of array-CGH has allowed precise mapping of the boundaries of the gained and lost regions, thereby helping to elucidate the clonal relationship between multifocal tumours (Shelley Hwang et al, 2004; Wa et al, 2005).
The study revealed that 79.6% of the chromosomal rearrangements occurred among neighboring regions in the folded chromosomes, based on mapping of the boundaries of the rearranged regions to the folded three-dimensional structures of the human chromosomes and the replication timing profiles.
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CEO of Professional Science Editing for Scientists @ prosciediting.com