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TopHat is a specially designed tool for fast splice-junction mapping of RNA-Seq reads.
Mapping of RNA-seq reads and transcript assembly and abundance estimation were conducted using Tuxedo Suite pipeline (TopHat v1.3.0/Bowtie v0.12.7/Cufflinks v1.0.3) and reported in Fragments Per Kilobase of exon per Million fragments mapped (FPKM).
The HPV status by mapping of RNA-Seq reads was concordant with the genomic, sequencing and molecular data, and indicated that 36 tumours were HPV and 243 were HPV (Supplementary Information section 1.2, Supplementary Fig. 1.1 and Supplementary Data 1.2).
VN performed mapping of RNA-seq data.
Additional file 7: Trimming and mapping of RNA-seq reads.
(B) Mapping of RNA-seq reads to RefBeet after applying Tophat.
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These amended genomes were then used as references for re-mapping of RNA-seq reads, with only the reads mapping uniquely and containing no mismatches used for further analysis.
Heat map of RNA-seq expression data from root, leaf, bud, flower and 3cm_fruit.
Additional file 12 gives the mapping results of RNA-seq reads.
By mapping millions of RNA-seq reads to individual transcripts, estimation of expression levels of individual exons and whole transcripts can be performed.
The PIK3CA gene definition in both Ensembl and RefGene, and the mapping profile of RNA-Seq reads were shown in Figure 6.
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