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Mosaik examines all possible mapping locations for each read.
Such clonal reads were identified on the basis of having identical mapping locations for both reads in a pair.
While MOSAIK aligning sequencing reads, an additional field inside the BAM file, the ZA tag, is then populated with information about the read mate, including MEI information, location, mapping quality and number of mapping locations for the mate.
Included in this release of the RMAP software package is functionality for mapping paired-end reads, making more sophisticated use of quality scores, collecting mapping locations for ambiguously mapping reads and mapping bisulfite-treated reads.
This article highlights a clear need for the development of more detailed statistical models that can incorporate knowledge of SNP variation into read-mapping and explicitly model uncertainty in the mapping locations for reads when testing for allele-specific effects.
RSEM allows a maximum of 2 mismatches per 25 bp seed but has Bowtie report all possible mapping locations for each read (reads that do not map are discarded).
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For the first question, the pre-condition for computing the GMS across the genome is that all possible reads should be considered at every possible starting position, and the correct mapping location for every read is known.
Identical mapping location for both 45 bp reads is unlikely to occur randomly, and for each set of clonal read pairs we only included a single read pair in the analysis.
The -u option was used to report uniquely mapped reads only, whereas -H option was used to breaks ties using Hamming distance when there was more than one best mapping location for a read, which would give the most accurate mapping results with little or no cost to the mapping percentage.
To control for these biases, we identified clonal molecules in the PE data using as a signature the mapping location for both reads in a pair (or if one read did not map uniquely, the first 10 bp of sequence was used as a signature).
More than a thousand are included, with line drawings, images and map locations for relevant buildings.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com