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The complete list of renamed markers can be found in Guo et al. (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, Submitted).
The total number of published SSR markers has now risen beyond the 2,847 cataloged in a related paper by Guo et al. (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, submitted) to around 6,000 [ 26].
SSR markers were genotyped on an ABI3730XL Capillary DNA Sequencer (Applied Biosystems, Foster City, CA) as described in a related paper by Guo et al. (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, Submitted) using forward primers labelled with FAM, HEX, or TAMRA fluorophores.
The same markers were used to create a map between two A. batizocoi accessions and to determine syntenic relationships between the A and B genome species (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, submitted).
Screening of the parental accessions with 2,138 SSR markers derived from A. hypogaea EST sequences resulted in 1,768 (82.7%) that were scorable (detected by ABI3730XL genotyping systems) and 896 (41.9%) that were polymorphic (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A- and B-genome diploid species of peanut, Submitted).
A set of 34 SSR markers from genomic sequences of Arachis previously screened for polymorphism between parents of the A. duranensis mapping population (Guo Y et al: Comparative mapping in intraspecific populations uncovers a high degree of macrosynteny between A-and B-genome diploid species of peanut, Submitted) were also mapped (Table 1).
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Six CRC modifiers previously mapped in intraspecific crosses were also replicated.
A genetic linkage map of 1705.1 cM across 20 linkage groups (LGs) was thereafter obtained, containing 237 markers, whose order was largely consistent with a previous map obtained in intraspecific crosses of N. furzeri [ 18].
Linkage group G8 has proved difficult to map in some intraspecific peach mapping efforts [ 29, 34].
Simple traits [ 17] and QTL for lifespan [ 18] were previously mapped in an intraspecific cross of short- and longer-lived N. furzeri strains.
To enrich the currently available SSR marker resources in peanut and gain clearer insights into karyotypic evolution within the genus, a new set of EST-SSR markers was developed and mapped in an intraspecific B-genome mapping population.
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