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In addition to the numerical ranking information, gene or protein identifiers were extracted and mapped to the most recent EntrezGene IDs using mapping files obtained from NCBI.
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Mapping files we obtained from Uniprot [ 30] and the NCBI gene databases.
Briefly, DB uses the Binary Alignment/Map (BAM) files obtained by mapping the paired-end read sequences to the human reference genome using BWA [ 24] (or any other alignment tool that can produce BAM files).
SAM files obtained from mapping were converted and visualized using SAMtools [41] and BamView [42].
We applied our pipeline to the BAM files obtained by mapping the FASTQ-formatted paired-end read data obtained from COLO-829 cell line to the human reference genome using BWA [ 24].
The candidate genes for each disease were mapped with the three drug-target association files obtained from the three drug databases, and the results retrieved.
We mapped the list of 1,497 candidate genes with drug-gene target association files obtained from the three drug databases.
The MapMan mapping file was obtained from [ 48]; 27837 of the 29549 genes on the microarray are present in the mapping file.
The MapMan mapping file was obtained from http://www.gomapman.org/; 27,212 of the 29,549 genes on the microarray were present in the mapping file.
The files, obtained by the A.C.L.U.
For comparison, the previous procedure was repeated using CM3.5, its gene annotation file, and a filtered vcf file obtained by mapping the high-quality re-sequencing reads to CM3.5 and processed using SAMtools and BCFtools.
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