Exact(6)
Identified Technologies claims it is improving drone mapping efficiency by 75%, allowing companies to acquire data faster.
This compares to 50.4percentt unique mapping efficiency by Bismark on bisulfite treated data according to Figure 6.
We found that recovering the reads improved unique mapping efficiency by 9-10% compared to the bisulfite reads.
We hypothesized that the hairpin sequencing technology can improve the mapping efficiency by recovering the original sequences, which have higher entropy.
For the undifferentiated data, recovering the untreated sequence improved unique mapping efficiency by an average of 9.15% (p-value = 0, variance≈ 10−8) compared to the average unique mapping efficiency of the T-enriched and the A-enriched reads.
Comparing the numbers of MP reads (123,511,268; Table 1) and MBD2-seq reads (329,882,382 for 2 libraries) it became clear that incorporation of MP reads into the reference improved mapping efficiency by only 0.85%, and 1.20%, respectively, for the two MEBS-PE libraries.
Similar(54)
This suggests that the hairpin sequencing strategy can improve mapping efficiencies by uniquely mapping one sequence to find the position of the other sequence.
Enriching the pool of reference sequences by including previously identified variant allele patterns, followed by remapping, improved mapping efficiency, raising coverage by 50% from regions with the greatest deviation, resulting in a coverage pattern indistinguishable from controls.
For the differentiating data, the unique mapping efficiency was improved by 10.59% (p-value = 0, variance ≈ 10−8).
PolyCat uses GSNAP's single-nucleotide polymorphism (SNP -tolerant mapping to minimize the mapping efficiency biaSNP -tolerantNPs between genomapping
Figure 7 shows the results, in terms of mapping efficiency (sensitivity), achieved by the different versions of HPG-Methyl for datasets with a mutation rate of 1% with respect to the reference genome.
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