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Therefore, the mapping cross described here allowed for the isolation of 178 independent haploid progeny.
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Mapping crosses are described in Ludwig et al. (1993).
The map position of notch3 st51 was refined by scoring SSLP markers from linkage group 3 (LG3) in notch st51 mapping crosses as described (Talbot and Schier, 1999).
(A ) Design of the mapping cross.
JJ oversaw development of mapping cross.
We therefore predicted 34 linkage groups per mapping cross.
A red fluorescent protein (RFP) marker was inserted at the MAT a locus in each of these mutant strains before crossing to the mapping strain described below to avoid diploid contamination when sorting haploid segregant progeny (FASTER MT approach; Chin et al. 2012).
Moretzsohn et al. [ 11] reported genetic linkage mapping for a single controlled cross of oil palm using random amplified polymorphic DNA (RAPD) markers and the pseudo-testcross mapping strategy described by Grattapaglia et al. [ 12].
To construct the second generation linkage map, a mapping population was obtained by artificial crossing, as described previously [ 25].
The QTL analysis of autosomes was performed using interval mapping as first described by Lander and Botstein [ 8] and later modified for analyzing crosses between outbred lines by Haley and co-workers [ 9, 10].
The findings of each case are compared to the findings of the other cases using cross-case comparison techniques and data displays (e.g., tables, causal network maps) as described by Miles and Huberman [ 73].
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