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To run MAQGene we used the general parameters previously described [12] setting "max sum of error qualities for mapping assembly and pileup" to 200.
The Genome Analysis Toolkit (GATK) was then used to generate a mapping assembly and to call SNPs for each strain.
Genome mapping, assembly and finishing was performed using CLC Genomics Workbench (version 6.5.0/6.5.1) including the CLC Microbial Genome Finishing Module (version 1.2.1/1.3.0) from Qiagen.
This combines mapping, assembly, and repeat analysis, and is coupled with a self-tuning k-mer strategy for the sensitive and specific discovery of InDels in exome capture data.
First, because of the sheer number of sequences, all kinds of sequence analyses, including database search, multiple alignment, sequence mapping, assembly and phylogenetic analysis, are getting more time consuming and memory demanding and require more manual efforts for parsing the output results.
Similar(55)
We first used reference-based iterative map assembly and then removed the Rmaps in these assembled optical map contigs from the whole Rmap dataset, the leftover or the uncontiged Rmaps were for de novo map assembly via G & G.
On this level, all of the pipelines we tested performed very well; the artefacts in the annotation data mostly originated from the re-mapping assembly and not from the annotation process itself.
TMS and SZ performed optical map assembly and analysis.
JZ worked on clone culture, data process, map assembly and drafted the manuscript.
High level of genome duplications would certainly add complexities for physical map assembly and possibly produce Q-clones.
SZ contributed to the design of the study, carried out partial data collection, performed optical map assembly and comparison between optical map and sequence, and drafted the manuscript.
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