Sentence examples for mapping and nucleotide from inspiring English sources
Mapping and nucleotide sequence analysis verified that the retrieved DNA sequence contained the promoter region and the initiating methionine of the murine CD146 gene, corresponding to the published CD146 cDNA sequence (Kohama et al., 2005).
All DNA constructs were analyzed by restriction enzyme mapping and nucleotide sequencing.
The resistance gene cluster in serovar Emek identified by PCR mapping and nucleotide sequencing contained SGI1-J3 whish integratedted in SGI1 at another position than the majority of SGI1.
In 1992, Nyström and Neidhardt [ 27] reported the cloning, mapping, and nucleotide sequencing of a monocistronic gene in Escherichia coli encoding a small (13.5 kDa) cytoplasmic protein with increased synthesis during growth inhibition or presence of toxic agents.
Complete genome sequences offer several advantages over restriction site maps and nucleotide sequences of targeted regions.
PCR-derived duplicate reads (judged by mapping location and nucleotide sequence) with lowest sum of base qualities were also discarded.
Read mapping and single nucleotide polymorphism (SNP) filtering were carried out as described previously [ 20, 29].
Successful gene cloning was verified by colony PCR, restriction mapping and direct nucleotide sequencing.
Its main supported analyses are: read cleaning, transcriptome assembly and annotation, read mapping and single nucleotide polymorphism (SNP) calling and selection.
Moreover, libraries were constructed in most cases with the aim of producing markers useful for genetic mapping, and di-nucleotide markers, being by far more frequent in the genomes and easy to isolate, were better suited for this scope.
We thank the reviewers for their suggestion and we have (1) made a table of sequencing depth of each sample as Supplementary file 1A; (2) made tables in Supplementary file 1B E that provide the number of mapped reads, RPKMs, and nucleotide coverage for the four classes of genes showing in Figure 1B, 1C, 1D, and 1E.
