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The Genomics Initiative focuses on gene mapping and microarray work.
SFP discovery and mapping was achieved using pseudo-testcross screening and selective mapping to simultaneously optimize linkage mapping and microarray costs.
Due to the vastly different levels of resolution afforded by cytogenetic mapping and microarray analysis, it is difficult to directly correlate the results of these two methods.
Detection of robust SFPs was improved by an initial SFP-discovery step based on the pseudo-testcross strategy followed by using a selective mapping approach to simultaneously optimize linkage mapping and microarray costs.
A combination of quantitative trait locus mapping and microarray analysis is a useful approach to reduce the overall effort needed to identify genes associated with quantitative traits of interest [ 31].
A combination of quantitative trait locus mapping and microarray analysis is a useful approach to reduce the overall effort needed to identify genes associated with quantitative traits of interest.
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Recently, the integration of QTL mapping and microarray-based genome-wide transcriptome profiling of parents and bulks of homozygous RILs has been found to be a powerful approach to narrow down the number of candidate genes underlying the QTLs of interest and for isolating the possible genes regulating the traits.
This indicates a similarity of gene coverage between whole sets of induced genes identified by EST mapping and microarrays.
Indeed, our quantitative comparison of gene profiling data using the Shannon index demonstrated that EST mapping and microarrays can efficiently complement each other.
Linkage and association studies as well as integrated analyses using genetic mapping and microarrays have revealed some genes that may be responsible for MD progression or resistance, such as GH, IFNγ and SULT [ 27].
As genes belonging to these groups tend to have different paralogs, better understanding of the results will require mapping contigs and microarray probes to the Spalax genome when it becomes available.
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