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To redress this shortcoming, we have developed forward genetics for Meloidogyne hapla permitting the identification, mapping, and isolation of genes based on phenotype alone.
Bacterial Artificial Chromosome (BAC) genomic libraries are source of large genomic DNA insert clones for sequencing projects, physical mapping and isolation of intact genes [ 10, 11].
Large-insert DNA libraries from pig have been used for in situ hybridization experiments, whole-genome physical mapping and isolation of genes and gene clusters as well as for the identification of regulatory elements.
Future work will be directed at further fine mapping and isolation of rnpm2, in conjunction with gene expression studies to identify genes controlling resistance and candidates for rnpm2 in the region of interest.
The information here gathered on the genotypic structure of NILs will be integrated in future experimental programs aimed at the fine mapping and isolation of major heterotic QTL, a crucial step towards the understanding of the molecular bases of heterosis in maize.
In fact, the integration of the high-density SNP markers identified within the QTL introgression regions and of the genomic information gathered on the architecture of these NILs will greatly improve the scope of future experimental programs aimed at the fine mapping and isolation of major heterotic QTL, a crucial step towards the understanding of the molecular bases of heterosis in maize.
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Most markers developed by these approaches are based on DNA or cDNA polymorphisms between wild species and cultivated tomato, which lead to the construction of the first generation reference linkage maps and isolation of genes of interests.
These methods and results will help develop more RGL-tagged molecular markers that can be used further for genetic mapping and the isolation of resistance genes from common bean in the future.
Knowledge of the GC content of a genome and codon usage is useful when devising PCR-based strategies for mapping and gene isolation, as well as for hybridization studies by microarray.
Next generation sequencing (NGS) technologies are providing new ways to accelerate fine-mapping and gene isolation in many species.
Several of such segregating populations have entered into a pipe-line of genetic mapping and R gene isolation in our laboratory [ 12, 14, 32, 36- 38].
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maps and isolation
mapping and single
mapping and pacing/distance
mapping and analysis
mapping and localization
mapping and location
mapping and traffic
mapping and navigation
mapping and unification
mapping and testing
mapping and measurement
mapping and cartography
mapping and foreperiod
mapping and correlation
mapping and motion
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