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Dadmanesh F, Fan X, Dastane A, Amin MB, Bose S. Comparative analysis of size estimation by mapping and counting number of blocks with ductal carcinoma in situ in breast excision specimens.
However, for mammalian genomes, there are technical challenges associated with mapping and counting short-tag sequences.
DESDE-LTC includes an alpha-numerical coding system, a glossary and an assessment instrument for mapping and counting LTC.
Expression levels were estimated by mapping and counting filtered RNA-seq data sets against the M1 draft genome using EDGE-pro pipeline (Magoc et al. 2013).
Instead, the students were provided with the files containing raw counts for reads corresponding to all maize genes for all genotype/condition combinations, essentially "skipping" tedious steps of read mapping and counting.
We first investigated a number of reference transcript collections in order to determine whether a comprehensive and accurate description of berry transcriptional profiles could be developed by mapping and counting the reads generated through Illumina sequencing against predicted mRNA transcripts.
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Castor bean gene abundance that included 31221 unigenes was used for mapping tags and counting the tag abundance.
We mapped and counted GFP-positive cells (endogenously SPIG1-positive cells), CTB-positive cells (retrogradely labeled cells from the MTN), and GFP·CTB-double-positive cells in the GCL in the flat-mount retina.
For each 2 Mb non-overlapping window used in Figure 1, the nucleotide sequence was randomised 1000 times, and MRSfinder was used to map and count the number of MRS in each randomised sequence.
Algorithmic predictions of mRNA transcriptomes based on this data and using the GAZE computational framework resulted in the prediction of 30,434 or 26,346 transcripts from the 8x and 12x genome assemblies, respectively, and provided the first two datasets to which we mapped and counted our sequencing reads.
They cover about 40% of the surface, but only 35% was photographed with the quality required for geological mapping and crater counting.
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