Sentence examples for mapping and alignment from inspiring English sources
The whole-genome sequence (WGS) data for each isolate was aligned to the most recent version of the C. immitis RS3 strain sequence (AAEC02000000) (6 ) by using the software program BFAST (7 ) with the following exclusion criteria: 1) indel-containing reads; 2) reads aligning to multiple locations; and 3) reads with mapping and alignment scores <20 and <100, respectively.
Transcript sequences were aligned to the reference genome using GMAP 2014 02-28 (Genomic Mapping and Alignment Program) [ 37] with the following parameters: −-intronlength = 350000, −-no-chimeras, −-canonical-mode = 1, −-cross-species.
Wu, T.D. & Watanabe, C.K. GMAP: a genomic mapping and alignment program for mRNA and EST sequences.
Integrated approaches to synteny mapping and alignment have been proposed, most of which operate on pairs of genomes [8], [25] [27].
The data was put through our sequencing pipeline consisting of base calling using the Illumina Pipeline, mapping and alignment with BWA [15], PCR duplication removal with SAMTools [16], and GATK [17] for variants calling.
Apart from greater ease-of-use, integrated synteny mapping and alignment methods could in theory provide more accurate inference because the alignment can influence the synteny map and vice-versa.
Similar results were obtained when the analysis was performed employing the hg19 release of the human genome reference database, confirming the consistency of mapping and alignments for these regulatory sequences across databases.
A minimum tiling path of single haplotype clones was selected based off of a fingerprint map and alignment of existing BAC end sequences (BES) to span the 1q21.1-q21.2 region of interest.
This integrated physical BAC map contains contigs constructed by fingerprinting and BAC end sequencing and is ordered using landmark maps and alignments with the human genome.
We improved BS-Seeker2 by utilizing multiple short-read mapping aligners, supporting gapped mapping and local alignment and building special indexes for handling RRBS data.
It has diverse applications ranging from lead prediction to automated reaction mapping and visual alignment of similar compounds.
