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(1) Mapping SNPs and Initializing Particles.
Following mapping, SNPs and small indels were identified based on the mpileup files generated by SAMtools [ 89].
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Mapping, SNP and small indel calling were performed using LifeScope 2.5.1 with standard parameters.
Much larger numbers will be required if the associated variants are less common, if the mapping SNP and fracture-associated SNP have different allele frequencies, or if gene-gene interactions are involved.
To validate these putative homeologies, and to identify other homeologous regions, sequences flanking mapped SNPs and MSVs were aligned against each other using BLAST [ 25] to identify paralogs.
To further develop our understanding of homeology within Atlantic salmon, sequences flanking mapped SNPs and MSVs were compared with the stickleback reference genome.
To our knowledge, only one study in Pinus taeda[ 30] reported LD for 807 mapped SNPs and confirmed the assumption of independence between genetically linked loci.
Significant segregation distortions were detected in 121 of the 887 mapped SNPs and seven of the sixteen mapping parents following FDR correction (mean corrected alpha of 0.003) (Additional file 20).
The genetic map presented here is the first-generation genetic map for black spruce, which provides a framework to map SNP and other markers in the second-generation genetic map.
The tables for gene mapping, SNP mapping and Human-Mouse alignment were obtained from ensemble, dbSNP, HGVBASE and UCSC and installed locally.
The tree confirmed that SL1344 was the closest finished reference to the S. Typhimurium 135@ isolates, and therefore the most suitable reference for read mapping, SNP calling and gene content comparison.
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