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This paper goes along the topical automated data science and focuses on developing the OCAM (Online Cognitive Automated Mapper) system that allows to automatically-build cognitive maps without turning to experts.
In combination with the analysis of laser altimetry data obtained from the NASA Airborne Topographic Mapper system and cosmogenic dating of granite boulders we show that the features previously thought to be shorelines are not horizontally or linearly continuous.
PFGE was carried out using a CHEF Mapper system (Bio-Rad, Hercules, CA) for 74 hours in 0.8% agarose, 1x TBE buffer at 14°C, with 35 minute pulse time at the field strength of 2 V/cm and 106 degree included angle.
Genomic DNA of yeast TAR clones was digested with NotI and separated on 1% agarose gels in 0.5 x TBE using a CHEF mapper system (BIORAD) with the following conditions: forward: 9 V/cm, 10 s initial switch, 40 s final switch, reverse: 6 V/cm, 10 s initial switch, 40 s final switch, for 24 hrs at 14°C.
The resulting restriction fragments were separated by PFGE using the MAPPER system (Bio-Rad Laboratory, HerCAles, CA, USA).
PFGE analysis was performed using a contour-clamped homogeneous electric field (CHEF) mapper system (Bio-Rad, USA), as previously described (8).
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E. aerogenes typing was carried out by pulsed field gel electrophoresis (PFGE) with a CHEF-Mapper system (Bio-Rad France) as previously described [53].
Plugs were loaded onto a 1% agarose gel and resolved with a CHEF-Mapper system (Bio-Rad LabOntarioes, Mississauga, Ontario).
Electrophoresis was performed using the CHEF-Mapper system (Bio-Rad, Marnes-la-Coquette, France) with the standard λ concatamer ladder (Bio-Rad) as a molecular size marker.
The sheared large DNA fragments were end-repaired with a DNA terminator kit (Lucigen) for 30 min at room temperature followed by heat inactivation at 70°C for 15 min. The end-repaired large fragment DNA was fractionated on a CHEF-Mapper system (BioRad) in 1% PFGE agarose, 0.5X TBE, using the following conditions: 6 V/cm, 0.1 - 2 s time ramp, 11 hrs, 120° angle, and 14°C.
For PFGE analysis, genomic DNA was embedded in agarose using the CHEF Genomic DNA Plug Kit (Bio-Rad), digested with the indicated restriction enzymes, and electrophoresed in 1% agarose using the CHEF Mapper XA system (Bio-Rad).
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