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The 114 Acinetobacter complex strains that were experimentally optically mapped were isolated from three US military hospitals over a period of 7.5 years.
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DNA from individual G978-18 × Azucena F2 plants selected for mapping was isolated from fresh leaves using the CTAB method (Murray and Thompson, 1980).
MAP was isolated from liver of five and spleen of two animals (Table 3).
Furthermore, MAP was isolated from a clinically diseased donkey (Stief et al. 2012).
MAP was isolated from 59 of the 186 individual samples (31.7%), representing 50 of the 62 MAP-positive pools (80.6%).
MAP was isolated from faeces collected from ELISA positive cattle [ 5], and tissues that had histological lesions of Johne's disease.
MAP was isolated from all the deer in this group by culture after 50 weeks post infection and 12 out of the 15 samples collected immediately before slaughter were seropositive using the Paralisa™ test.
The BES reads from which the 126 mapped markers were isolated represent 88 unique BAC FPC contigs.
More recently two MAP strains were isolated from the blood of T1DM patients by our group [4].
Furthermore, the T1DM patients from whom the MAP strains were isolated (23 and 27), were also both positive for all the serological tests used except for Gsd (antibodies for which were not abundant in the plasma of patient no. 27); this is a further indication of the accuracy of the tests that we employed.
Markers for map construction were isolated from our genomic libraries [ 7].
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