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FPKM (Fragments per Kilobase of exon per Million fragments mapped) were calculated for each gene, but only transcripts showing an FPKM > 1 were kept for further analysis.
Here number of reads mapped were calculated by mapping reads on assembled transcripts using CLC Genomics Work bench with a mismatch, insertion, deletion cost of 2, 3 and 3 respectively.
The reads of each of the four libraries (N-, N, N + and NO3) were mapped onto the 36,781 unigenes and the RPKM values (reads per kilobase of exons per million of fragments mapped) were calculated for all the unigenes present in the libraries.
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Cortical thickness maps were calculated for each subject.
Spatially inclusive and comprehensive indicator maps were calculated for the entire country (83,872 km2).
Susceptibility maps were calculated using frequency shifts in gradient echo MR images acquired at 9.4 T.
Climatic parameters (MAT, CMMT, WMMT, MAP) were calculated using bioclimatic analysis.
The local resolution maps were calculated by ResMap (Kucukelbir et al., 2014).
Electron density maps were calculated using PHENIX (Adams et al., 2010).
For this purpose, T1 0) maps were calculated from the five T1-weighted scans for each animal.
The solar potential raster maps were calculated from the r.sun model.
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