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HiSeq2000 100-bp reads were mapped to version 1.1 of the switchgrass genome (www.phytozome.org).org
Weighted Mapped Read Counts: Transcriptome v3.6: the number (and percentage) of reads mapped to version 3.6 of the An.
Resequencing data were mapped to version 2.2 of the D. miranda MSH22 reference assembly (GenBank: AJMI00000000.2) using bowtie2 (Langmead and Salzberg, 2012).
To identify genes corresponding to reads in each library, the purity-filtered reads were mapped to version 2 of the maize B73 reference genome (AGPv2) [ 16] with the Short Oligonucleotide Alignment Program 2 (SOalignerigner (Additional files 2 and 3) [ 17].
After trimming adapters, the clean reads were mapped to version 2.1 of the reference genome (available via http://phytozome.jgi.doe.gov/pz/portal.html#!info alias=Org_Sbicolor) using the BWA program [ 25], allowing a maximum of five mismatches and disabling long gaps in the mapping procedure.
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For this study oligonucleotide probes and genes were mapped to Flybase version FB2008.06.
Reads were mapped to UCSC version mm9 using Bowtie (Langmead et al., 2009).
Those that do not map, will move to the second step, where they will be mapped to a version of the human genome, hg19, with exonic sequence masked out.
The unigenes were mapped to UniProt version 14.8 (minus genomic sequences from Vitis vinifera, which have not been annotated) using BLASTx analysis with an expectation highly expressed value of e < 10-5 included in the high-scoring segment pair.
To assess whether mRNA-Seq could be used to detect quantitative differences between the abundance of transcripts originating from the A and C genomes of resynthesised B. napus, we analysed the numbers of reads mapped to each version (A genome-cured and C genome-cured) of the unigenes in the cured reference.
The three data sets are: A set of three whole genomes recommended [ 10] for benchmarking purposes belonging to a Central European (CEU) trio (NA12878, NA12891 and NA12892) from the Thousand Genomes project [ 11] were downloaded by FTP as aligned BAM files (approximately 71× coverage, mapped to HG19 version of the human genome) from the European Bioinformatics Institute [ 12].
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