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Only 135 of the 193 tag types were relevant (Table 4) with 48 tag types that mapped ambiguously to more than one location in the Homo Sapiens transcriptome/genome, and another 10 tag types that mapped to Mus musculus transcriptome/genome. Mus musculus mappings may be an indication of minor contamination of the in vivo LNCaP Hollow Fiber model samples with host (mouse) RNA.
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Over 80% of reads mapped to the Mus musculus genome (GRCm38) and over 60% to exons (mapping overview in Figures S1G and S1H).
Raw data was downloaded from the NCBI Gene Expression Omnibus and the reads were mapped to the Mus musculus genome (mm9) and transcriptome (RefSeq, downloaded on 07/17/2009) as previously described (Lienert et al., 2011).
FastQ reads were mapped to the Mus musculus genome version 9 (mm9) using Bowtie2 with default parameters, apart from an adjustment to relax the gap extension penalty (option: −-rdg 5,1).
The resulting 50-mer reads were mapped to the NCBI7/mm9 (Mus musculus) genome assembly using Bowtie v. 0.12.8 with standard settings for unique mapping [ 32].
The transcriptional responses between species were similar despite the fact that the P. berghei were not synchronous and the read depth was much lower for P. berghei owing to contaminating mouse mRNA, as shown by the high percentage of reads mapping to Mus musculus (Additional file 6).
The sequence reads were quality filtered and mapped to the mouse genome (Mus musculus, mm9 assembly, [ 52]) using Bowtie [ 53] with the following settings (−n 2 –e 70 –m −1-k 1).
The reads were mapped to the reference genome [ Mus musculus UCSC mm10 sequence], and the alignment files were generated as BAM files.
FastQ files were converted into fastA files with the FASTQ-to-FASTA converter and split into pools according to barcodes using the FASTQ/A Barcode splitter (both tools were from http://hannonlab.cshl.edu/fastx_toolkit/), trimmed to 35 bases and mapped to the mouse using the Mus musculus NCBI Built 37.1.
Sequence reads were mapped to the NCBI37 (mm9/July 2007) release of the entire mouse (Mus musculus) genome using BFAST 0.7.0a (Homer et al., 2009a, 2009b).
The pre-processed reads were mapped to the P. falciparum 3D7 2013-3-1 reference genome, or P. berghei ANKA 2013-3-1 and musculusulus build 38 reference genomes (P. berghei experiments) using Bowtie2 and TopHat2 programs.
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