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The obtained sequences were then mapped to mouse genome using UCSC genome browser for splice junction analyses.
The only gene mapped to mouse chromosome 12 and human chromosome 14 with polymorphisms associated with azoospermia is MLH3, which causes a meiotic arrest [51].
Predicted hairpins were then mapped to mouse exons from UCSC known genes and Ensemble data sets and matched to the corresponding Affy IDs.
For BioGRID, modules were also identified from human datasets and the genes were mapped to mouse using best-reciprocal BLASTP hit analysis.
To find support for miRNA expression, we downloaded all ESTs and and cDNAs mapped to mouse genome assembly NCBI 36 from the UCSC Genome Browser Database [61] in September 2006 and processed the mappings as described in [71] to remove low-quality sequences, reliably assign sequences to a genomic strand, and merge sequences obtained from the same cDNA clone.
Coordinates of miRNA precursor sequences mapped to mouse genome were obtained from miRBase release 12 [ 41].
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The Dp(10 1Yey mouse model (Dp10) of DS is trisomic for orthologs of 39 Hsa21 protein-coding genes that map to mouse chromosome 10 (Mmu10), including four genes with known sex differences in functional properties.
More than seven million CAGE tags have been sequenced from 145 mouse cDNA libraries and mapped to the mouse genome [56].
The resulting alignments were overlaid to, in this order, NCBI's RefSeqs [ 89], mouse mRNA, and human proteins mapped to the mouse genome by UCSC [ 79].
The Allen Mouse Brain Atlas (Atlas) probes have been previously mapped to the mouse (mm8) genome [ 36].
Reads were mapped to the mouse genome (mm9) using Bowtie2 and uniquely mapped reads were used for further analysis.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com