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Sequence reads were mapped to maize B73 genome (version 3) (Schnable et al. 2009).
The remaining reads were mapped to maize genome using Bowtie [ 25] with no more than one mismatch.
Unique small RNA sequences were mapped to maize genome (B73 releasev2 (release 5a.57 in May, 2010)) reference sequences by SOAP [ 70].
Transcripts represented on the Affymetrix GeneChip® Maize array were mapped to maize transcripts with a custom Perl script that used the DNA sequences provided by Affymetrix and BLASTn to query the ZmB73 4.53a filtered CDS sequences [ 31].
On average 74% of the total reads mapped to maize inbred B73 reference genome sequence (Additional file 1: Table S1), with most of the reads mapping to exons (~96%) and only a small proportion mapping to introns (~1.8%) and intergenic regions (~2%; Additional file 2: Figure S1).
The 15 nt upstream and 5′ end of the reads that mapped to maize contigs were extracted to generate 30-sequence tags, which were used to align to newly identified miRNAs and miRBase (Release19.0, August ,2012) using the Cleave and pipeline [ 52].
Similar(54)
Of the 296 unigenes, 198 were mapped to the maize physical map using blastn.
Sequences were mapped to the maize genome (B73 RefGen_v2) to find clusters of sRNA.
Eight Activator (Ac) transposable elements mapped to the maize chromosome arm 1S were assessed for Ac transposition rates.
All high quality reads were mapped to the maize B73 reference genome [RefGen_v2; [ 21, 22] allowing large gaps of up to 50 kb to span introns.
In brief, unique reads ranging from18-25 nt were collected and mapped to the maize genome (B73 releasev2 (release 5b. 60 in February 2011)) reference sequences [ 62] by SOAP2 [ 37].
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