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We therefore mapped the breakpoints of the deficiencies more precisely both genetically by complementation analysis and molecularly by single embryo PCR.

Scanning the EST14 intron and ETV1 flanking region by long-range PCR and sequencing mapped the breakpoints in G270 ∼1.9 Kbp upstream of ETV1 exon 1 and ∼5 Kbp downstream of EST14 exon 1.

Since the P-element insertion was not the likely cause of the failure of Df 2R 03072 to complement xen72−3, we mapped the breakpoints of the deficiency region using lethal complementation tests between Df 2R 03072 and genes in the Lobe chromosomal region.

We have recently mapped the breakpoints of a t(1 2)(q41;p25.3) associated with isolated bilateral renal agenesis.

Kohno and Yokota (2006) mapped the breakpoints of 21 lymphoid leukemia cases, 21 lung cancer cases, and 32 other solid cancer cases at a 10-kb resolution.

We initially mapped the breakpoints by hybridization of amplified flow sorted derivative chromosomes onto the whole genome tiling path array held at the Wellcome Trust Sanger Institute [Fiegler et al., 2003].

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Similar(53)

This probe-signal pattern mapped the breakpoint to the 17q24.3 region enclosed between probes RP11-387O17 and RP11-304I14 (Figure 3A, B).

The 18q breakpoint in the patient 263/96 was mapped by FISH to a 140 Kb region defined by BAC RP11-496G14, thybridizedized to chromosome 18 and to der18 and by BAC RP11-850021 thybridizedized to chromosome 18 and to derX (not shown): genome sequence analysis mapped the breakpoint distal from the GALR1 gene and proximal to the SALL3 gene (Fig. 1a).

We used PCR to fine-map the breakpoints and amplify a junction fragment of 630 bp.

The in silico CNV detection algorithms used in the present study showed ambiguity in mapping the breakpoints.

We used the CREST method (Wang et al., 2011) to detect and map the breakpoints of the somatic rearrangements among the 14 HPR patients.

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