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Therefore we mapped the CDK9-binding region along AF4.
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Using a site-directed mutagenesis approach, Martin et al [29] have mapped the ERp57 binding site to tip of the P-domain (residues Glu239, Asp241, Glu243 and Trp244).
We mapped the Pcr1 binding sites in cells prior to and after H2O2 treatment using the same method as in the analysis of Atf1 binding sites.
Next, we mapped the Smad3-binding site on the BRCA1 protein by using HEK293T cell lysates that were transfected with Flag-Smad3 plasmid and a series of GST-BRCA1 protein fragments [20].
We mapped the H3 binding activity to the C-terminus of MTA1 and MTA2.
Prior to CD4 gp120 crystal structures, mutagenesis studies mapped the gp120 binding sites on CD4.
We next mapped the Lis1 binding site on dynein using single-particle image processing.
We have mapped the ERAL1-binding site to a 33 nucleotide section delineating the 3′ terminal stem loop region of 12S mt-rRNA.
Recently, a crystallographic analysis mapped the AMA1-binding site of RON2 to a C-terminal sequence comprised between two closely spaced transmembrane domains [ 46].
In addition, we have mapped the WT1 binding sites and investigated the effect on WT1 binding activity of individual ZF deletions and Denys-Drash syndrome point mutations to this target.
In order to identify the genes controlled directly by GlnR and thus forming the GlnR regulon, we mapped the 53 binding sites onto the profile of transcripts regulated by GlnR during nitrogen limitation, using the Integrated Genome Viewer [ 35, 36], examples of which can be seen in Figure 2 (all 53 binding sites in nitrogen limitation can be viewed in Additional file 3: Figure S2).
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