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Once the target aggregation was identified, the study area was marked and the nest entrances mapped, resulting in a 3.5 m×2 m study area with 10 nest entrances (Fig. 1).
Only loci that were polymorphic within one or both parents could be mapped, resulting in 3,430 putative SNPs to be analyzed further (Table 2, Figure 1).
For the "twice" and "union" sets, larger number of canonical pathways were mapped resulting in a larger number of mapped genes.
In particular, for heterozygous SNPs, the allele concordant with the reference is more likely to be mapped, resulting in a bias towards the reference allele and an inflated rate of false positive ASE.
A total of 817,470,439 reads (59,643,456,282 bases) were aligned to approximately 97% of the reference genome (53,783,377,648 bases were uniquely mapped), resulting in an average of 24.7X sequence coverage of the horse's genome (Additional file 3, Table S2).
Although only 70% of the diagnostic categories could be mapped to a specific condition in the ICNARC Coding Method, the hierarchical nature of the ICNARC Coding Method enabled most of the remaining diagnostic categories to be mapped to a higher level in the hierarchy, and only 4% of diagnostic categories were unable to be mapped resulting in the exclusion of 1.3% of admissions.
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Approximately half of the 6222 VR genes that we investigated could be successfully resolved, and those that were unambiguously mapped resulted in an extremely accurate dataset.
These analyses are performed using mapped results in the SAM format generated by the basic analysis.
Instead, the algorithms lend themselves for vertical mapping resulting in area-efficient pipeline structures.
In the same year, Kikkawa and Suzuki [7] generalized the Kannan mapping resulting in the following theorem.
From this two dimensional plane, we then take the color map, resulting in a common 2-D image.
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