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The chromosomal deletion found in the karyotypically stable ESC clone used for this study (clone 7 30) was previously mapped on mouse chromosome 16 (36071173–36166797, NCBI mouse Build 37) by inverse-PCR [1].
Remarkably, genes encoding type 1 (e.g. stefins) and type 2 (e.g. cystatins) cystatins are grouped in two main clusters mapped on mouse chromosome 16 and 2, respectively (Table 1), with the exception of Cstb on chromosome 10 and Cst6 on chromosome 19.
Additional genetic studies, such as the chromosomal deletion of the complete stefin gene cluster mapped on mouse chromosome 16 or the cystatin gene cluster mapped on chromosome 2, would be required to potentially uncover and dissect compensation mechanisms that might be occurring in the mutant mouse model presented here.
All sequenced reads were mapped on mouse transcript references converted from refFlat's GTF file using the gffread command in Cufflink.
Reads were mapped on mouse reference genome mm9 using TopHat version 2.0.4, using default parameters and UCSC annotation (http://genome.ucsc.edu/).ucsc.edu/
We first collected probes for genes for which we knew where they mapped on mouse chromosomes – Nancy: – these were the anchors: several anchors for each chromosome.
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To prepare a tandem duplicate-free mouse genome we considered 19 684 known genes from the LocusLink database that were mapped on the mouse genome assembly (NCBI build 32) [ 57].
Clones which were not caught by any of these filters were mapped on the mouse genome (release UCSC mm5 [2]) using the sim4 [37] program.
IDs are mapped on their mouse Ensembl gene ID (version 41).
The LAM-PCR products sequenced by 454-pyrosequencing were mapped on the mouse genome (mm9) using a dedicated bioinformatics pipeline.
For evaluation of the intergenic sORFs, reads were first mapped on the mouse cDNA database (Ensembl version 66).
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