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For alignments to the IAdb, Novoalign (http://www.novocraft.com/) was used (version 2.05.20 [-o SAM -r A -R 0, default options] (http://www.novocraft.com/) which reports all locations and genomes mapped equally well by a given read pair.
Moreover, reads that mapped equally well to more than 40 genomic locations were discarded.
Multireads that mapped equally well to more than 20 genomic locations were discarded.
Reads that mapped equally well to the positions of two or more mature miRNAs were divided equally to the read counts of those mature miRNAs.
A maximum of two mismatches were allowed and reads that mapped equally well to more than 40 genomic locations were discarded.
If a read mapped equally well to multiple locations (therefore producing a mapping score of zero), MAQ randomly returned one of the locations.
Similar(52)
One drawback of using uniquely-mapped RNA-seq reads for expression analysis is that any read which maps equally well to identical regions in different genes is discarded, potentially resulting in an underestimation of the expression levels of highly similar genes [ 21].
Reads mapping equally well to two positions were assigned randomly.
However, both sequences may map equally well to both positions in base space.
Reads mapping equally well to both genotype-specific references were assigned to a "both" category.
Overall, about 87 91 % of reads uniquely map to genomic regions, while approximately 3.5%% of reads map equally well to multiple locations.
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