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Williams said she reviewed government and business records and mapped at least a dozen metal plating, casting, polishing and finishing facilities and other potential sources of hexavalent chromium within a few blocks of where regulators detected the high readings.
Partial reads were 76 bp in length and mapped at least 30 bp to the region.
Notably, we found that more than 97% of the sequence reads were mapped at least once onto genomic objects identified via the automated annotation Microscope pipeline.
If we required only a single uniquely mapping peptide (rather than requiring at least two peptides mapped, at least one of which was unique), we still only observed 72 of the 483 previously identified proteins.
Mapped reads with ≥95% similarity and ≥85% of the length matching the assembled sequence, and reads that aligned at least once within a range of 1,000 to 5,000 nt of each other, were considered in agreement with the master circle assembly and 9,134 reads mapped at least once within this range covering the entire assembled genome.
We selected probes that could be unambiguously mapped at least once to either the genome (where there was an annotated transcript) or to the reference transcriptome, with the main requirement being that there is an association to an official gene symbol.
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A total of 498 markers (384 of which were DArTs) were in common, i.e. they were mapped in at least two mapping populations.
All CDSs mapped against at least one SAS and 1,218 mapped against at least one pair of RNA-seq reads (87% of the total).
All pathways mapped to at least one CMM and all modules mapped to at least one pathway appear in the Heat maps.
If targeted sequences come from the same line as the genome used for mapping, all should be mapped to at least one locus.
Both methods mapped 0.7% and 1.2% pairs discordantly (mapped positions at least 500 bp apart).
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